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5:02

Sequencing-based Approaches for Profiling DNA Methylation

DNA methylation plays an important and dynamic role in regulating gene expression. DNA methylation is essential for normal development and is associated with a number of key processes including genomic imprinting, X-chromosome inactivation, suppression of repetitive elements, and carcinogenesis. Utilizing the highly trusted, proven DNA analysis platforms in the industry, CD Genomics will assist you in the epigenome wide association studies, combining the comprehensive, expert-selected coverage and high-throughput compatible with large sample size. https://www.cd-genomics.com/DNA-Methylation-Microarray-Service.html

3 years ago 27 views

6:42

Principle and Workflow of Whole Genome Bisulfite Sequencing - CD Genomics

Epigenetic studies have confirmed that DNA-methylation modification of specific gene regions plays an important role in chromosome conformation and gene expression regulation. Methylation of DNA cytosine residues at the C5 (5meC) is a common epigenetic mark in many eukaryotes and is widely found in CpG or CpHpG (H=A, T, C). There are mainly three approaches, including endonuclease digestion, affinity enrichment, and bisulfite conversion (Table 1). Almost all sequence-specific DNA methylation analysis approaches require a methylation-dependent treatment before amplification or hybridization to maintain fidelity. Various molecular biology techniques, such as next-generation sequencing (NGS), are subsequently performed to detect 5meC residues. https://www.cd-genomics.com/principles-and-workflow-of-whole-genome-bisulfite-sequencing.html

3 years ago 23 views

9:28

The Development and Workflow of RNA-seq

CD Genomics has been providing the accurate and affordable RNA-Seq (RNA sequencing) service for decades. We combine both Illumina (short reads) and PacBio (long reads) platforms to obtain the transcriptome that allows de novo assembly or re-sequencing for bacteria, plants, animals and humans. https://www.cd-genomics.com/RNA-Seq-Transcriptome.html

3 years ago 13 views

Bioinformatics Analysis of 16s rRNA Amplicon Sequencing Data

6:04

12:49

Microbial Genomics

Until recently, the properties and compositions of the microbiota in the planet are still largely a black box. Next generation sequencing (NGS) has proven to be an invaluable tool for investigating diverse environmental and host-associated microbial communities, helping to generate enormous new data sets that can be mined for information on the composition and functional properties of vastly great numbers of microbial communities. https://www.cd-genomics.com/Microbial-Genomics.html

3 years ago 18 views