Unequivocal Discrimination of Leucine and Isoleucine in De Novo Antibody Sequencing

Based on our proprietary technology for de novo sequencing of antibodies and our expertise in protein mass spectrometry and informatics, our standard procedures include the following steps:

De Novo Antibody Sequencing WorkflowFig. 1 De Novo Antibody Sequencing Workflow.
Antibody protein is initially digested into overlapping peptides using various trypsin
Digestion with LC-MS/MS, then fragmentation optimization
Overlapping peptides are characterized by de novo peptide sequencing
Automated antibody sequence assembly using characterized peptides
Data analysis is performed using state-of-the-art computational algorithms that efficiently derive the amino acid sequence of the digested peptide
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