Mutant Parasites for Use as Vaccines and Platforms for Screening for Compounds

**Must See: This is f*cking Crazy! Here is another unbelievable Head Shaker and guess who owns this wonderful patent? The NIH.

The patent was just last year in 2024.

Our 3 letter health agencies have gone mad and they are supposed to be our regulators looking out for our best interests.

**NOTE: This is probably an indication that they are going to infect the population with parasites so we all can be “TREATED.”

In my personal opinion, utilizing my freedom of speech -- I think that vaccines have been used to introduce diseases and infections that can create conditions to be treated with other vaccines. That is how evil Big Pharma is and the regulators are in bed with them. And only the very tip top leaders of all the organizations are in the know.

Our 3 letter agencies have gone way past the rogue stage to sick, demented, careless evildoers.

🧬 Invention Overview

This patent, filed by Indiana University and assigned in part to the NIH (filed June 4, 2010; published June 6, 2013) , discloses:

1. Genetically engineered protozoan parasites, such as Toxoplasma gondii, modified to express a non-phosphorylatable form of the translation initiation factor TgIF2α (e.g. S71A mutation) or be deficient in eIF2α kinases.

2. Uses for these mutants:

Live attenuated vaccines—offering immunization by infection with weakened parasites.

Drug screening platforms—identifying compounds that inhibit the eIF2α phosphorylation mechanism essential for parasite survival.
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🔬 Biological Basis & Targeted Pathway

In T. gondii, eIF2α phosphorylation is critical for survival under stress (e.g., when extracellular, outside host cells).
The kinase TgIF2K–D (plus A, B, C isoforms) phosphorylates eIF2α. Mutants lacking kinase or phosphorylation (e.g., TgIF2α-S71A) become significantly less viable when stressed.

These altered parasites retain abilities such as cell invasion and replication but cannot effectively survive outside a host, reducing their virulence .
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🧪 Key Invention Claims

1. Mutant Parasite Strains

Altered TgIF2α (e.g., S71A) or deletion of TgIF2K genes limits phosphorylation, diminishing extracellular fitness and virulence .

2. Vaccine Applications

Attenuated parasites can be used to vaccinate mammals (e.g., sheep, cats, cattle, humans), offering safer immune priming than wild-type strains .

3. Drug Discovery Platforms

These mutants can be used to screen therapeutic compounds that target eIF2α phosphorylation or the kinases (e.g., TgIF2K-D).

Applicable across Apicomplexa parasites (e.g., Toxoplasma, Plasmodium, Cryptosporidium) due to kinase homology.

4. Anti-infective Methods

Approaches include either chemical inhibition of parasitic kinases (e.g., using salubrinal) or genetic disruption to reduce infectivity .
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🔄 Experimental Evidence
Genetic Construction: Creation of the S71A TgIF2α allele and TgIF2K-D knockouts in T. gondii.

Phosphorylation Analysis: S71A mutants fail to phosphorylate eIF2α under stress (e.g., calcium ionophore treatment), via immunoblotting .

Fitness Tests: Mutants show reduced viability—especially outside host cells—while retaining host cell invasion and replication skills .

Vaccine Proof-of-concept: In vivo delayed onset of acute toxoplasmosis in mice infected with mutants suggests protective potential .
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⚙️ Technical Highlights

Phosphorylation Site Mutation: Substitution of serine 71 with alanine (S71A) blocks TgIF2α phosphorylation.

Kinase Gene Knockouts: Targeted removal of kinases (e.g., TgIF2K-D) disrupts phosphorylation pathways.

Drug Screening: Deploy mutants to test compounds that prevent eIF2α phosphorylation—impairing parasite stress response.

Vaccine Strategy: Live-attenuated vaccine using mutants that replicate but cannot effectively spread under stress.
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💡 Potential & Impact

New Vaccine Candidates: Offers a promising whole-parasite vaccine for toxoplasmosis in humans and animals.

Therapeutic Discovery Tool: Enables focused screening on the eIF2α pathway—a conserved vulnerability across related parasites.

Broader Scope: The approach may generalize to malaria (Plasmodium) and cryptosporidiosis (Cryptosporidium).
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✅ Conclusion

US 2013/0142829 A1 presents a sophisticated approach to controlling T. gondii and similar parasites:

By genetically attenuating their stress-response pathway (eIF2α phosphorylation), parasites remain alive yet non-lethal—ideal for vaccines.

These mutants also serve as robust screening platforms to discover anti-parasitic drugs targeting stress-response mechanisms.

With demonstrated in vitro and in vivo viability, the invention bridges basic mechanistic insight with practical medical applications.

Main Question?!?👇

❓Does the vaccine prevent infection or just symptoms?

This live-attenuated parasite vaccine (based on Toxoplasma gondii mutants) is primarily designed to:

Allow infection with a weakened form of the parasite,
But prevent disease by stopping the parasite from spreading or causing damage in the body.
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🔬 Mechanism in Detail:
The genetically engineered parasites can still invade host cells and replicate early on.

However, they lack the ability to survive stress (like being outside cells or facing immune attacks) due to disrupted eIF2α phosphorylation.

Because of this, the mutant parasites:
Die off more quickly,
Don’t disseminate as well,
Don’t cause severe symptoms.
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So what does the vaccine do?

Effect Explanation

Infection Yes — you’re exposed to live mutant parasites.
Symptoms / Disease No — they’re too weak to cause real illness.

Immunity Yes — the immune system “learns” from the weakened parasite and is ready for real infection.
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Bottom Line:

The vaccine does not completely prevent infection, but it prevents illness and severe outcomes. It's similar to how some live-attenuated vaccines (like for yellow fever or polio) work — you get a weak “version” of the pathogen that trains your immune system without making you sick.

Access patent here:

Mutant Parasites for Use as Vaccines and Platforms for Screening for Compounds
https://patents.google.com/patent/US20130142829A1/en

Source: Jay Wilson -- https://www.facebook.com/jay.wilson.977829/videos/1140671081118028
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