Cell Proliferation Assay

Measure of cell proliferation based on the metabolic activity of a population of cells can be used. Tetrazolium salts or Alamar Blue are compounds which become reduced in the environment of metabolically active cells, forming a formazan dye that subsequently changes the color of the media, which is caused by increased activity of the enzyme lactate dehydrogenase during proliferation. The absorption of the media-containing dye solution can be read using a spectrophotometer or microplate reader in low- or high-throughput configurations.Show more

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