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Step to Step DNA Isolation/Extraction Protocol From Pure Culture | Molecular Biology Lab Techniques
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Step to Step DNA Isolation/Extraction Protocol From Pure Culture | Molecular Biology Lab Techniques
oin us in this informative video as we demonstrate the step-by-step process of extracting DNA from a bacterial culture. Learn the essential techniques and reagents involved in isolating DNA, including centrifugation, lysis, precipitation, and purification. Our easy-to-follow tutorial will equip you with the necessary skills to extract DNA for a wide range of scientific applications. Discover the fascinating world of molecular biology and unlock the secrets hidden within the DNA of bacteria. Watch now and embark on your journey into the world of genetic research!
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We provide microbiology lectures for the students to educate people in easy way. To provide e-learning experience and good note on microbiology subjects. This video includes:
Content:
Chemical:
1. 1x TE
- Tris 10mM
- EDTA 1mM
- pH 8
2. 10% SDS
- 10g in 100ml of distilled water
3. Phenol Chloroform Isoamyl-alcohol
Phenol 25ml
Chloroform 24ml
Isoamyl-alcohol 1ml
4. Chloroform isoamyl-alcohol
- Chloroform 24ml
- Isoamyl-alcohol 1ml
5. 5M NaCl
- 292.5g NaCl in 1000ml of distilled water
6. 10% CTAB
- NaCl 4.1g
- CTAB 10g
- Distilled water 100ml
.................................................................................................................................................................
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