iTRAQ-based Proteomics Analysis
Proteomics research involves the identification and characterization of proteins to elucidate their function and interactions with other proteins. Since the composition of protein mixtures may vary across different cell types and may change under certain physiological conditions, one of the goals is often to quantify the up- or down-regulation of individual proteins. Shotgun proteomics approaches enable identification of proteins that are up-regulated or down-regulated under specific conditions and this can be studied in different cell and tissue lysates. Isobaric tags for relative and absolute quantification (iTRAQ) make it possible to both identify and quantify proteins simultaneously. iTRAQ can easily be multiplexed, enabling analysis of up to 8 different samples within the same experiment,that allows for high-throughput quantitative proteomics analysis. https://www.creative-proteomics.com/services/itraq-based-proteomics-analysis.htm
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