what is elisa and its procedure and principle

Introduction of Elisa……………………………………………………4
Material required………………………………………………………5
Principle ………………………………………………………………..6
Procedure ………………………………………………………………7
Indication of presence of antigen…………….......…….......................16
Indication of presence of antibody………………....……....................17
Advantages and disadvantages of competitive Elisa……...................18
Spectrophotometry……………………………………………………19
Elisa data interpretation ………………………………………………20
Applications of Elisa …………………………………………………..21