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Estimate d-spacing from TEM_HRTEM & Indexing SAED pattern using Gatan Digital Micrograph Software
High-resolution transmission electron microscopy (HRTEM) is identical to transmission electron microscopy (TEM) imaging, except that the magnifications used are sufficiently high to easily observe the lattice spacing of inorganic materials (typically on the order of several Å). Although such lattice spacings can easily be recorded onto the film at moderate magnifications (the 3.4 Å spacing between graphite layers can be recorded onto the film at magnifications as low as 15,000-20,000x), CCD cameras and much higher magnifications (usually above 200,000 or 300,000 × at the plane of the imaging device) allow the user to control the defocus much more easily and produce the best HRTEM images. Visualization of these atomic spacings requires a more stable goniometer, brighter electron source, and more stable electronics than those found in most electron microscopes not designed for HRTEM. Virtually all recently built intermediate-voltage instruments (i.e., electron microscopes operating at 200, 300, and 400 kV) meet the necessary requirements for HRTEM, and it is more often specimen and environmental issues that prevent such instruments from recording images with atomic resolution information.
SAED ( Selective Area Electron Diffraction) Patterns from TEM uses Parallel electron beams from the aperture for obtaining information about the crystallinity of the material. (single crystal, poly crystal and amorphous).
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