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In this video I shift more conic sections and this time shift Hyperbolas. Once again this procedure is the same as in my earlier videos on shifting ellipses (and circles) and parabolas. Nonetheless it is important to see the same procedure applied to different functions. Shifting a parabola with the center at the origin so that the center is now at the point (h , k) requires switching every (x , y) point on the original hyperbola with (x – h, y – k). Thus to obtain the new (x , y) values we need to add h to every x – h value, and k to every y – k value. In other words, we shift every point horizontally by h and vertically by k. This is a great video to reiterate the concept of shifting functions in general so make sure to watch this video! Download the notes in my video: https://1drv.ms/b/s!As32ynv0LoaIh5YHzNNtJk4zVRcWHQ View video notes on the Hive blockchain: https://peakd.com/mathematics/@mes/shifted-conics-hyperbolas Related Videos: Shifted Conics: Parabolas: https://youtu.be/UlrAkAW3T8Y Shifted Conics: Ellipses (and Circles): https://youtu.be/ZqtUfaQbj7U Equation of a Circle and it's proof: http://youtu.be/xMXYJ9UeF4I Conic Sections: Hyperbolas: Example 2: Vertical Hyperbola: https://youtu.be/zBNSTkoEzFI Conic Sections: Hyperbola: Definition and Formula: https://youtu.be/UBIHovXNV9U Conic Sections: Parabolas: Definition and Formula: https://youtu.be/kCJjXuuIqbE Conic Sections: Ellipses: Definition and Derivation of Formula (Including Circles): https://youtu.be/9dETsJ2tz_M Hyperbola - Definition and derivation of the equation: x^2/a^2 - y^2/b^2 = 1: http://youtu.be/Y6iYC4VEAi0 Hyperbola Examples: http://youtu.be/LvccUMFrY3k . ------------------------------------------------------ SUBSCRIBE via EMAIL: https://mes.fm/subscribe DONATE! ʕ •ᴥ•ʔ https://mes.fm/donate Like, Subscribe, Favorite, and Comment Below! Follow us on: MES Truth: https://mes.fm/truth Official Website: https://MES.fm Hive: https://peakd.com/@mes MORE Links: https://linktr.ee/matheasy Email me: contact@mes.fm Free Calculators: https://mes.fm/calculators BMI Calculator: https://bmicalculator.mes.fm Grade Calculator: https://gradecalculator.mes.fm Mortgage Calculator: https://mortgagecalculator.mes.fm Percentage Calculator: https://percentagecalculator.mes.fm Free Online Tools: https://mes.fm/tools iPhone and Android Apps: https://mes.fm/mobile-apps

In this video I go over the main assumption in the main paper on COVID-19 "virus" isolation, and that is the RNA extracted from patient and cell culture supernatants is assumed to be "viral". It took me a long time before realizing this since the paper does not state this directly. Instead, I had to ask ChatGPT to clarify a paragraph from the paper, as well as double-checking with the mainstream science literature. And indeed this is a key assumption made in virology in general. Ribonucleic acid (RNA) is supposedly the genetic makeup of the new SARS-CoV 2 "virus" which supposedly causes "COVID", so when a "viral infection" is suspected, then typically the assumption is that RNA found is from this "virus". ChatGPT also outlines several methods to "confirm" this assumption, such as using PCR to detect specific RNA sequences, comparing the sequences with known "viral sequences", and "immunological assays". These assays use "antibodies" (or immune system "defense proteins") that are supposedly specific to and bind to proteins (called antigens) on the "virus"; thus allowing for indirect identification of this "virus". All of this is interesting, yet it still remains highly questionable that a bunch of nucleotide sequences (aka RNA) are somehow assumed to be "infectious disease causing particles that can infect and replicate through a host and spread to other individuals (and even shut down the entire world)"... The paper is listed below: - A Novel Coronavirus from Patients with Pneumonia in China, 2019 - Authors: Na Zhu et al - Published on January 24, 2020 in The New England Journal of Medicine (NEJM) - https://www.nejm.org/doi/full/10.1056/NEJMoa2001017 The timestamps of key parts of the video are listed below: - RNA Assumed to be from a "Virus": 0:00 - RNA extracted from Airway Fluid Samples and Culture Supernatants: 0:22 - Asking #ChatGPT to Explain Paragraph: 0:48 - #ChatGPT: Assumption is RNA is from a "Virus": 1:41 - Summary of RNA "Virus" Assumption: 3:21 This video is taken from my earlier video listed below: - 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8 - Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper - Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx Related Videos: 🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist . ------------------------------------------------------ SUBSCRIBE via EMAIL: https://mes.fm/subscribe DONATE! ʕ •ᴥ•ʔ https://mes.fm/donate Like, Subscribe, Favorite, and Comment Below! Follow us on: MES Truth: https://mes.fm/truth Official Website: https://MES.fm Hive: https://peakd.com/@mes MORE Links: https://linktr.ee/matheasy Email me: contact@mes.fm Free Calculators: https://mes.fm/calculators BMI Calculator: https://bmicalculator.mes.fm Grade Calculator: https://gradecalculator.mes.fm Mortgage Calculator: https://mortgagecalculator.mes.fm Percentage Calculator: https://percentagecalculator.mes.fm Free Online Tools: https://mes.fm/tools iPhone and Android Apps: https://mes.fm/mobile-apps

In this video I go over the main COVID-19 "virus" isolation paper's methods on the techniques used in sequencing this "new virus". The first step in sequencing the "virus" is assuming that the RNA found in the patient airway fluid samples and cell culture supernatants somehow belong to a "virus". After this assumption, a combination of Illumina sequencing and nanopore sequencing were used to somehow produce a "virus genome". After this, PCR tests were designed with specific primers to test for this new "virus". The "virus" sequences were then compared with reference sequences. And finally, statistical analysis was performed on determining the likely evolutionary history of the "virus" genome. Essentially, the paper outlines a lot of obscure computer and statistical techniques to form long genetic sequences from RNA assumed to be from a "virus". Truly bizarre "science"... The paper is listed below: - A Novel Coronavirus from Patients with Pneumonia in China, 2019 - Authors: Na Zhu et al - Published on January 24, 2020 in The New England Journal of Medicine (NEJM) - https://www.nejm.org/doi/full/10.1056/NEJMoa2001017 The timestamps of key parts of the video are listed below: - Paper Methods: Sequencing the "Virus": 0:00 - RNA as a Template to Clone and Sequence Genome: 0:37 - Illumina Sequencing, Nanopore Sequencing: 1:13 - Sequence Reads Assembled into Contig Maps: 1:46 - Contig, Contiguous, Contig Map: 2:00 - Primers Designed for PCR to Fill Genome Gaps: 3:11 - Primer, 5' or 3', Complementary DNA (cDNA), Sanger Sequencing: 3:44 - Multiple-Sequence Alignment of New "Virus": 6:19 - Multiple-Sequence Alignment, Phylogenetics, Bootstrap Replicates: 6:41 - Summary of Sequencing the "Virus": 7:30 This video is taken from my earlier video listed below: - 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8 - Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper - Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx Related Videos: 🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist . ------------------------------------------------------ SUBSCRIBE via EMAIL: https://mes.fm/subscribe DONATE! ʕ •ᴥ•ʔ https://mes.fm/donate Like, Subscribe, Favorite, and Comment Below! Follow us on: MES Truth: https://mes.fm/truth Official Website: https://MES.fm Hive: https://peakd.com/@mes MORE Links: https://linktr.ee/matheasy Email me: contact@mes.fm Free Calculators: https://mes.fm/calculators BMI Calculator: https://bmicalculator.mes.fm Grade Calculator: https://gradecalculator.mes.fm Mortgage Calculator: https://mortgagecalculator.mes.fm Percentage Calculator: https://percentagecalculator.mes.fm Free Online Tools: https://mes.fm/tools iPhone and Android Apps: https://mes.fm/mobile-apps

In this video I go further into the main paper on COVID-19 "virus" isolation and this time review the paper's methods on transmission electron microscopy (TEM) preparation. TEM involves firing a beam of electrons through an ultrathin section and the resulting interactions with the sample forms an image that can then be made visible through fluorescent screens or other methods. But to get to this point, the sample is typically first mixed with many heavy metal chemicals and embedded into a resin block. This means that any biological samples will be dead and heavily processed. For this paper, the first sample prepared was the supernatant of the "infected" human airway cell cultures that showed "cytopathic effects" (CTE), and was then "virus inactivated" with a chemical preservative and ultracentrifuge at around 100,000 RPM. The resulting sedimented "virus" particles was then heavy metal stained on film-coated metal grids. The second sample was the "infected" airway cell cultures (that showed CTE) themselves, and they were "fixed" with heavy metal preservatives, dehydrated with alcohol, and then embedded in a resin block before being cut to tiny nanometer slices for TEM. This is a very complex process to somehow see dead particles assumed to be "virions"... The paper is listed below: - A Novel Coronavirus from Patients with Pneumonia in China, 2019 - Authors: Na Zhu et al - Published on January 24, 2020 in The New England Journal of Medicine (NEJM) - https://www.nejm.org/doi/full/10.1056/NEJMoa2001017 The timestamps of key parts of the video are listed below: - Paper Methods: Electron Microscope Preparation: 0:00 - Virus Inactivation, Paraformaldehyde, Fixation: 0:49 - Ultracentrifuge, Transmission Electron Microscopy (TEM), Heavy Metals: 1:41 - Fluorescence, Electrons, Electromagnetic Radiation (EMR), Aether: 2:29 - TEM Schematic: 3:22 - Enriched Supernatant Was Negatively Stained: 4:18 - Negative Stain, Staining, Film-Coated Grids, Hydrophilic, Formvar Grid: 5:06 - Fixing Airways Cells Showing Cytopathic Effects: 6:22 - Glutaraldehyde, Osmium Tetroxide, Grade Ethanol: 6:54 - PON812 Resin, Viscosity, Embedding: 7:31 - Ultrathin Sections Cut from Resin Block: 8:27 - Nanometers, Uranyl Acetate, Lead Citrate: 8:51 - Summary of Electron Microscope Preparation: 9:10 This video is taken from my earlier video listed below: - 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8 - Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper - Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx Related Videos: 🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist . ------------------------------------------------------ SUBSCRIBE via EMAIL: https://mes.fm/subscribe DONATE! ʕ •ᴥ•ʔ https://mes.fm/donate Like, Subscribe, Favorite, and Comment Below! Follow us on: MES Truth: https://mes.fm/truth Official Website: https://MES.fm Hive: https://peakd.com/@mes MORE Links: https://linktr.ee/matheasy Email me: contact@mes.fm Free Calculators: https://mes.fm/calculators BMI Calculator: https://bmicalculator.mes.fm Grade Calculator: https://gradecalculator.mes.fm Mortgage Calculator: https://mortgagecalculator.mes.fm Percentage Calculator: https://percentagecalculator.mes.fm Free Online Tools: https://mes.fm/tools iPhone and Android Apps: https://mes.fm/mobile-apps

In this video I go over further into the main paper on COVID-19 "virus" isolation, and this time review the paper's methods in isolating the "novel virus". The stated methods involved first collecting the lung fluid samples in a plastic cup, and then chemical preservatives (PBS) or fetal bovine serum (FBS) were added. The samples were then centrifuged and the resulting supernatant was assumed to somehow contain the "virus" and used to "infect" human airway cell cultures obtained from other lung cancer patients. Then the cultures were "incubated" or grown for 2 hours at 37°C, and then diluted to a new cell culture. This procedure was done 3 times total and each time the cell cultures were monitored using optical light microscopy for "cytopathic effects" (or cell breakdown / death). Cultures showing cytopathic effects were then prepared for electron microscopy. No information on the procedure for controls were presented. And no explanation as to why the centrifuged supernatant of the patient lung fluid samples were assumed to contain the "virus". The paper is listed below: - A Novel Coronavirus from Patients with Pneumonia in China, 2019 - Authors: Na Zhu et al - Published on January 24, 2020 in The New England Journal of Medicine (NEJM) - https://www.nejm.org/doi/full/10.1056/NEJMoa2001017 The timestamps of key parts of the video are listed below: - Paper Methods: Isolating the "Virus": 0:00 - Viral Transport Medium (VTM), Acids and Bases : 1:14 - Fetal Bovine Serum (FBS): 3:08 - Samples Centrifuged, Supernatant Use to Infect: 3:57 - Centrifugation, Supernatant, Cancer: 4:31 - Cells Grown on Plastic Surface: 7:01 - Plastic substrate, Expanding Cells, Passage-1 Cells, Plating: 7:29 - Cells Cultures Grown in Air-Liquid Interface: 9:42 - Air-Liquid Interface (ALI), Cellular Differentiation: 10:04 - Stem Cells, Respiratory Epithelium, Cilia: 12:57 - Apical Cells Washed with PBS: 14:14 - Apical, Microlitre: 15:08 - MES Note: Where is the "Virus"?: 16:13 - 2-hour Incubation, Unbound "Virus" Washed Away: 16:40 - Incubator, 37° C, Serial Passage: Cultural Dilution: 18:16 - Cells Monitored for Cytopathic Effects: 19:12 - Light Microscopy, Cytopathic Effect: 19:54 - Summary of Isolating the "Virus": 20:34 This video is taken from my earlier video listed below: - 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8 - Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper - Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx Related Videos: 🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist . ------------------------------------------------------ SUBSCRIBE via EMAIL: https://mes.fm/subscribe DONATE! ʕ •ᴥ•ʔ https://mes.fm/donate Like, Subscribe, Favorite, and Comment Below! Follow us on: MES Truth: https://mes.fm/truth Official Website: https://MES.fm Hive: https://peakd.com/@mes MORE Links: https://linktr.ee/matheasy Email me: contact@mes.fm Free Calculators: https://mes.fm/calculators BMI Calculator: https://bmicalculator.mes.fm Grade Calculator: https://gradecalculator.mes.fm Mortgage Calculator: https://mortgagecalculator.mes.fm Percentage Calculator: https://percentagecalculator.mes.fm Free Online Tools: https://mes.fm/tools iPhone and Android Apps: https://mes.fm/mobile-apps

In this video I go over a review of the "Viral" Diagnostic Methods from the main paper on COVID-19 "virus" isolation. The paper states that 4 lower respiratory tract samples were collected from Wuhan pneumonia patients (of "unknown cause") in December 2019 and were compared with 7 samples from Beijing pneumonia patients (of "known cause"), yet this comparison is never properly detailed in the paper. Nucleic acids were extracted from the samples and then polymerase chain reaction (PCR) was used to test for "22 known viruses and 4 known bacteria". Next generation sequencing (NGS) technology was then performed and they somehow detected sequences not discovered by the PCR tests. Reverse transcription PCR (RT-PCR) was then used to detect "viral RNA", which later in the paper they conclude is from a novel "coronavirus". This appears more as an exercise in computer generated sequencing than actual detection of real-life "disease causing particles"... The paper is listed below: - A Novel Coronavirus from Patients with Pneumonia in China, 2019 - Authors: Na Zhu et al - Published on January 24, 2020 in The New England Journal of Medicine (NEJM) - https://www.nejm.org/doi/full/10.1056/NEJMoa2001017 The timestamps of key parts of the video are listed below: - Viral Diagnostic Methods: 0:00 - Respiratory Tract, Bronchoalveolar-lavage : 1:14 - Extraction of Nucleic Acids: 2:02 - Cell Cultures, Negative Controls: 2:36 - PCR to Detect "Viruses" and Bacteria: 3:17 - Polymerase Chain Reaction (PCR), PCR cycles, dNTPs, Primers: 3:57 - NGS to Detect New Sequences, RT-PCR to detect "viral" RNA: 6:47 - Unbiased High-Throughput Sequencing or Next Generation Sequencing (NGS) 7:20 - Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR): 8:40 - Pan B-CoV, RNA-dependent RNA polymerase (RdRp): 9:40 - Summary of Detecting the "Virus": 11:25 This video is taken from my earlier video listed below: - 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8 - Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper - Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx Related Videos: 🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist . ------------------------------------------------------ SUBSCRIBE via EMAIL: https://mes.fm/subscribe DONATE! ʕ •ᴥ•ʔ https://mes.fm/donate Like, Subscribe, Favorite, and Comment Below! Follow us on: MES Truth: https://mes.fm/truth Official Website: https://MES.fm Hive: https://peakd.com/@mes MORE Links: https://linktr.ee/matheasy Email me: contact@mes.fm Free Calculators: https://mes.fm/calculators BMI Calculator: https://bmicalculator.mes.fm Grade Calculator: https://gradecalculator.mes.fm Mortgage Calculator: https://mortgagecalculator.mes.fm Percentage Calculator: https://percentagecalculator.mes.fm Free Online Tools: https://mes.fm/tools iPhone and Android Apps: https://mes.fm/mobile-apps