Distance From a Point to a Plane + Proof
In this video I derive the formula for the distance between a point to a plane in 3D coordinates. The distance formula can be derived by taking the scalar projection of a vector from a point on the plane to the desired point and projecting it onto the normal vector perpendicular to the plane. We can then utilize the geometric interpretation of the dot product to get a formula involving cosine and our desired distance. Further algebraic simplification yields the distance formula.
The timestamps of key parts of the video are listed below:
- Example 8: Distance from a Point to a Plane: 0:00
- Distance = Absolute Value of Scalar Projection: 1:15
- Distance Formula: 10:20
This video was taken from my earlier video listed below:
- Equations of Lines and Planes: https://youtu.be/qWQz6qPhXR8
- Video notes: https://peakd.com/hive-128780/@mes/equations-of-lines-and-planes
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0FO3u0IupqllNNkZTsffpIV
Related videos:
Vectors and the Geometry of Space video series: https://www.youtube.com/playlist?list=PLai3U8-WIK0FjJpwnxwdrOR7L8Ul8VZoZ .
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Intersection of Planes Forms a Line
In this video I show that two non-parallel planes form a line of intersection and show how to determine its equation. I also show the reverse procedure as well, that is, if we are given an equation of a line, then we can determine the equations of 2 planes that intersect to form this. This is readily evident when we are given the symmetric (or parametric) equations of a line. I also graph out the many equations of intersecting planes derived from a single line using the amazing GeoGebra 3D graphing calculator: https://www.geogebra.org/calculator/kttfn3pk
The timestamps of key parts of the video are listed below:
- Note 1: Two Linear Equations Can Represent a Line: 0:00
- Graphing Four Equations of Planes with GeoGebra 3D Graphing Calculator: 5:17
- Symmetric Equations of a Line = Two Planes: 6:40
- Note 2: Line of Intersection from Two Parametric Planes: 7:59
- Graphing Five Planes Intersecting to form One Line: 10:32
This video was taken from my earlier video listed below:
- Equations of Lines and Planes: https://youtu.be/qWQz6qPhXR8
- Video notes: https://peakd.com/hive-128780/@mes/equations-of-lines-and-planes
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0FO3u0IupqllNNkZTsffpIV
Related videos:
Vectors and the Geometry of Space video series: https://www.youtube.com/playlist?list=PLai3U8-WIK0FjJpwnxwdrOR7L8Ul8VZoZ .
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Parallel and Non-Parallel Planes + Example
In this video I go over parallel and non-parallel planes and go over an example on finding the angle between two non-parallel planes. Two planes are parallel if their normal vectors are parallel. If they are not parallel then they intersect in a straight line and form an angle between their 2 normal vectors. I go over an example on determining the angle between 2 non-parallel planes as well as finding the symmetric equations of their line of intersection. And as always, I double check my work using the amazing GeoGebra 3D graphing calculator, which you can play around with here: https://www.geogebra.org/calculator/kttfn3pk and here: https://www.geogebra.org/calculator/kttfn3pk.
The timestamps of key parts of the video are listed below:
- Parallel Planes: 0:00
- Example 7: 4:18
- Solution to 7a: Angle Between Planes: 4:42
- Graphing with GeoGebra 3D Graphing Calculator: 9:15
- Solution to 7b: Symmetric Equations of Line of Intersection: 9:57
- Graphing with GeoGebra: 18:55
This video was taken from my earlier video listed below:
- Equations of Lines and Planes: https://youtu.be/qWQz6qPhXR8
- Video notes: https://peakd.com/hive-128780/@mes/equations-of-lines-and-planes
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0FO3u0IupqllNNkZTsffpIV
Related videos:
Vectors and the Geometry of Space video series: https://www.youtube.com/playlist?list=PLai3U8-WIK0FjJpwnxwdrOR7L8Ul8VZoZ .
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Linear Equation of a Plane + Examples
In this video I go over the linear equation of a plane, which involves rewriting and simplifying the scalar equation of a plane. I do this by starting with the scalar equation of a plane and then collecting all like terms and replacing the constants addition with a single letter "d", thus obtaining the linear equation of a plane in the form: ax + by + cz + d = 0.
I also do 2 examples on planes to illustrate this concept. The first example involves finding the equation of a plane when given 3 points on the plane. The second example involves finding the point of intersection of a line and plane, whose equations are both given. And as always, I graph out the results using the amazing GeoGebra 3D graphing calculator as a double check of my work. You can play around with the calculator via these links: https://www.geogebra.org/calculator/mm6t7n4q and https://www.geogebra.org/calculator/raqsvnad.
The timestamps of key parts of the video are listed below:
- Linear Equation of a Plane: 0:00
- Example 5: Determine Equation of a Plane 3:18
- Graphing Plane with GeoGebra 3D Graphing Calculator: 11:28
- Example 6: Find Point At Intersection of Line and Plane 12:46
- Graphing with GeoGebra: 17:20
This video was taken from my earlier video listed below:
- Equations of Lines and Planes: https://youtu.be/qWQz6qPhXR8
- Video notes: https://peakd.com/hive-128780/@mes/equations-of-lines-and-planes
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0FO3u0IupqllNNkZTsffpIV
Related videos:
Vectors and the Geometry of Space video series: https://www.youtube.com/playlist?list=PLai3U8-WIK0FjJpwnxwdrOR7L8Ul8VZoZ .
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Vector and Scalar Equations of a Plane
In this video I go over planes in 3D and derive the vector equation of a plane and then convert that into scalar equations of a plane. A plane can be described by a point on the plane and a vector perpendicular to the plane. This perpendicular or orthogonal vector is called a normal vector. We can obtain a formula for the plane by noting that the dot product is equal to zero for perpendicular vectors. And then we can take a vector on the plane and towards the point on the plane and compute the dot product with the normal vector to obtain the vector equation of a plane. If we expand this vector equation by writing out its components and expanding out the dot product, we obtain the scalar equation of a plane.
I also go over an example on determining the equation of a plane, and as usual use the amazing GeoGebra 3D graphing calculator to graph out the plane. You can play around with the GeoGebra calculator here: https://www.geogebra.org/calculator/yrgywmbr
The timestamps of key parts of the video are listed below:
- Vector Equation of a Plane: 0:00
- Scalar Equation of a Plane: 6:02
- Example 4: 8:02
- Graphing Plane with GeoGebra 3D Graphing Calculator: 13:38
This video was taken from my earlier video listed below:
- Equations of Lines and Planes: https://youtu.be/qWQz6qPhXR8
- Video notes: https://peakd.com/hive-128780/@mes/equations-of-lines-and-planes
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0FO3u0IupqllNNkZTsffpIV
Related videos:
Vectors and the Geometry of Space video series: https://www.youtube.com/playlist?list=PLai3U8-WIK0FjJpwnxwdrOR7L8Ul8VZoZ .
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🍕#PizzaGate Part 32: MES vs. Reddit Saga + Tamera Luzzatto Rabbit Hole + Comet Ping Pong Hacker
In #PizzaGate Part 32 I go over my recent Saga with Reddit’s r/Conspiracy in which I was banned and then unbanned; as well as illustrate how the Tamera Luzzatto rabbit hole has deepened. I had initially been working on this video several weeks ago and it was supposed to be on the *alleged* hacker that found “Cheese Pizza” aka CP on Comet Ping Pong’s server but then I realized there were many more background topics to cover first, such as the *alleged* threat by James Alefantis towards PizzaGateGearRyan and also just the overall background context on James Alefantis and the strangely powerful Alefantis family lineage. But in the making of that video I got notified by Reddit’s r/Conspiracy forum that I was BANNED from the forum! So then I was working on that video, and in the middle of that video I was notified by an Insider with close ties to the Moderators at r/Conspiracy, and whom I like to call my very own “QAnon”, that the ban was actually due to a misunderstanding which then was resolved peacefully! Thus I deleted the video that I was making while literally in the middle of it.
When I got banned, initially I thought it was due to my post about the infamous Tamera Luzzatto “pool entertainment” email and connected DISTURBING baby blogs “Evie’s Cribs” and “Jack’s Place”. I thought this because the Luzzatto email is literally avoided by EVERY MSM outlet. Also given my previous experience with getting banned from r/911Truth for promoting the truth of 9/11, which is #FreeEnergy; see #911Truth Part 3, I thought censorship was just rearing its ugly head once again. And especially since the moderator that banned me from r/911Truth is also a former r/Conspiracy moderator! But it turns out that the likely scenario was actually due a misunderstanding of r/Conspiracy’s new “Rule 13: Submission Statement” and Reddit’s “Self-Promotion” Guidelines. And combined with me taking it to Twitter right away without messaging the moderators first, I had angered a few people within the forum. But luckily my QAnon helped all to understand each other’s position, and I have since apologized and deleted my Tweet regarding the ban.
Reddit is a GIANT website, thus it is important to make a presence there to the message you want heard. That is why even though I am unbanned, I will still be running my newly created r/ConspiracyMES forum to serve as what a conspiracy forum should be! I also have created v/ConspiracyMES on Voat because, as I argue in the video, and the Baphomet symbolism suggests, Voat likely won’t be that censorship-resistant if it had as much traffic as Reddit…
Moving on, I’ve learned that the Tamera Luzzatto rabbit hole deepens! New potentially BOMBSHELL info such as the “Evie’s Crib” disturbing tags (such as Baby Ambien (Sedative), Public Intoxication, Parental Manipulation, Tranquilizers”), possible Occult lineages, and Luzzatto praise of Democrat Senator Al "Groping" Franken, means I will be investigating further!
Stay Tuned for #PizzaGate Part 33…
Video Notes: https://peakd.com/@mes/pizzagate-part-32-mes-vs-reddit-saga-tamera-luzzatto-rabbit-hole-comet-ping-pong-hacker
Related Videos:
View full #Pizzagate series: https://peakd.com/pg/@mes/videos
#PizzaGate Part 31: Hollywood Victims Speak Out! #PedoWood + Weinstein + Mind Control: https://hive.blog/pizzagate/@mes/pizzagate-part-31-hollywood-victims-speak-out-pedowood-weinstein-mind-control
#PizzaGate Part 29: Pool Entertainment Luzzatto Email + WikiLeaks + 9/11 + MORE: https://hive.blog/pizzagate/@mes/video-notes-pizzagate-part-29-pool-entertainment-luzzatto-email-wikileaks-9-11-more
#911Truth video series: https://peakd.com/truth/@mes/911
#Occult video series: https://mes.fm/occult-playlist .
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Example on Skew Lines: Lines that Aren't Parallel and Don't Intersect
In this video I go over an example on determining whether 2 lines with given parametric equations are skew lines; which are lines that do not intersect and are not parallel. By looking at the direction vectors of each line, I note that the given lines are not parallel because their vector components are not scalar multiples of each other (aka they are not proportional). If the lines are not skew then they would have a point of intersection, in which case there would then exist a point (x, y, z) that is on both lines. If we then equate the corresponding coordinate parametric equations, we should get a solution. But in this case, we get a contradiction in one of the equations, thus we are dealing with skew lines! I also graph out the lines using the GeoGebra 3D graphing calculator and indeed they do not intersect.
The timestamps of key parts of the video are listed below:
- Example 3: Skew Lines: 0:00
- Solution to Example 3: 0:45
- Graphing Lines with GeoGebra: 10:10
This video was taken from my earlier video listed below:
- Equations of Lines and Planes: https://youtu.be/qWQz6qPhXR8
- Video notes: https://peakd.com/hive-128780/@mes/equations-of-lines-and-planes
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0FO3u0IupqllNNkZTsffpIV
Related videos:
Vectors and the Geometry of Space video series: https://www.youtube.com/playlist?list=PLai3U8-WIK0FjJpwnxwdrOR7L8Ul8VZoZ .
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Vector Equation of a Line Segment
In this video I go over the vector equation of a line segment instead of the entire line. I first start off with an example involving parametric equations and noting that setting the parameter t = 0 gets the first point on the line, while setting it to t = 1 gets the second point. From this fact, we can start off with our usual vector equation of a line and then describe it using our desired starting and ending vector, which point to the 2 positions of a line we want. Then we can just impose the interval of the parameter t to be from 0 to 1 to get our desired line segment!
The timestamps of key parts of the video are listed below:
- Parametric Equations of a Line Segment Example: 0:00
- General Vector Equation of a Line Segment: 5:32
- Summary of Equation of a Line Segment: 11:58
- Note on t = 0 and t = 1: 12:40
This video was taken from my earlier video listed below:
- Equations of Lines and Planes: https://youtu.be/qWQz6qPhXR8
- Video notes: https://peakd.com/hive-128780/@mes/equations-of-lines-and-planes
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0FO3u0IupqllNNkZTsffpIV
Related videos:
Vectors and the Geometry of Space video series: https://www.youtube.com/playlist?list=PLai3U8-WIK0FjJpwnxwdrOR7L8Ul8VZoZ .
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Direction Numbers and Symmetric Equations of a Line
In this video I go over what direction numbers and symmetric equations of a line are. Direction numbers are the components of the parallel vector in a vector equation of a line. We can use any three numbers that are proportional to the direction numbers and still get the same line. This is because the vector and parametric equations of a line are not unique.
The symmetric equations involve taking out the parameter t from each of the three parametric equations and then equating them to get equations that are symmetric, as the name implies. If one of the direction numbers is 0, then the symmetric equations can be simplified by having that coordinate equal to the corresponding point on the line.
I also go over an example on finding the parametric and symmetric equations of a line that passes through the points (2, 4, -3) and (3, -1, 1) and then determine the point it intersects the xy-plane. And as usual, I graph out the line using the amazing Desmos 3D graphing calculator: https://www.geogebra.org/calculator/cwrevexn
The timestamps of key parts of the video are listed below:
- Direction Numbers: 0:00
- Symmetric Equations of a Line: 3:40
- Symmetric Equations if a Direction Number is Zero: 7:00
- Example 2: 9:21
- Solution to 2(a): 9:46
- Solution to 2(b): 15:34
- Note on Example 2: 20:30
This video was taken from my earlier video listed below:
- Equations of Lines and Planes: https://youtu.be/qWQz6qPhXR8
- Video notes: https://peakd.com/hive-128780/@mes/equations-of-lines-and-planes
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0FO3u0IupqllNNkZTsffpIV
Related videos:
Vectors and the Geometry of Space video series: https://www.youtube.com/playlist?list=PLai3U8-WIK0FjJpwnxwdrOR7L8Ul8VZoZ .
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Parametric Equations of a Line
In this video I convert the vector equation of a line into its parametric equations form. To do this I first expand the vector equation by writing out its components and then summing them up. Then I equate each x, y, and z coordinate with its corresponding components resulting in 3 equations. These 3 equations are called the Parametric Equations of a line. I also go over an example on finding the vector and parametric equations of a line that passes through the point (5, 1, 3) and is parallel to the vector [1, 4, -2]. Finally, I graph the line using the amazing GeoGebra 3D graphing calculator, which you can play around with here: https://www.geogebra.org/calculator/wszwnpme
The timestamps of key parts of the video are listed below:
- Parametric Equations of a Line: 0:00
- Example 1: 4:31
- Solution to 1(a): 4:57
- Solution to 1(b): 13:03
This video was taken from my earlier video listed below:
- Equations of Lines and Planes: https://youtu.be/qWQz6qPhXR8
- Video notes: https://peakd.com/hive-128780/@mes/equations-of-lines-and-planes
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0FO3u0IupqllNNkZTsffpIV
Related videos:
Vectors and the Geometry of Space video series: https://www.youtube.com/playlist?list=PLai3U8-WIK0FjJpwnxwdrOR7L8Ul8VZoZ .
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Vector Equation of a Line
In this video I go over the vector equation of a line in 3D space. The method is similar to that of a line in 2D using the point-slope method. In 2D, a line is determined when a point on the line and the line's slope is known. Similarly, a line in 3D can be determined using vectors when a point on the line and a vector parallel to the line is known. The parallel vector can be multiplied by a scalar so that we can then find any point on the 3D line.
The timestamps of key parts of the video are listed below:
- Line in the xy-Plane: 0:00
- Vector Equation of a Line: 1:17
- Parameter t: 6:49
This video was taken from my earlier video listed below:
- Equations of Lines and Planes: https://youtu.be/qWQz6qPhXR8
- Video notes: https://peakd.com/hive-128780/@mes/equations-of-lines-and-planes
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0FO3u0IupqllNNkZTsffpIV
Related videos:
Vectors and the Geometry of Space video series: https://www.youtube.com/playlist?list=PLai3U8-WIK0FjJpwnxwdrOR7L8Ul8VZoZ .
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Vectors and the Geometry of Space: Equations of Lines and Planes
In this video I go over equations of lines and planes in 3D coordinates. This section is part of my calculus book chapter titled Vectors and the Geometry of Space. In 2D coordinates, a line can be described by a point on the line and its slope or direction. Similarly, a line in 3D coordinates can be described by a point on the line and a vector parallel to it which thus serves as the direction. A plane in 3D coordinates is described by a point on the plane and a vector perpendicular to the plane. The plane equation utilizes the fact that the dot product of two perpendicular vectors is equal to zero. Furthermore, the cross product is also used in determining the perpendicular vector, termed a normal vector. I go through several related concepts, such as scalar and vector equations of lines, segments of lines, and linear equations of planes, as well as many examples to give a thorough examination of 3D lines and planes.
The topics covered as well as their timestamps are listed below.
- Introduction: 0:00
- Calculus Book Reference: 1:13
- Sections in Calculus Book Chapter: 1:27
- Topics to Cover: 2:28
1. Lines: 3:42
- Vector Equation of a Line: 5:00
- Parametric Equations of a Line: 12:50
- Example 1: 17:22
- Solution to 1(a): 17:49
- Solution to 1(b): 25:55
- Direction Numbers: 28:08
- Symmetric Equations of a Line: 31:49
- Example 2: 37:29
- Solution to 2(a): 37:54
- Solution to 2(b): 43:43
- Note on Example 2: 48:38
- Equation of a Line Segment: 50:24
- Example 3: 1:04:16
2. Planes: 1:15:15
- Vector Equation of a Plane: 1:15:32
- Scalar Equation of a Plane: 1:21:13
- Example 4: 1:23:14
- Linear Equation of a Plane: 1:29:40
- Example 5: 1:32:58
- Example 6: 1:42:27
- Parallel Planes: 1:48:18
- Example 7: 1:52:34
- Solution to 7(a): 1:52:58
- Solution to 7(b): 1:58:13
- Note 1: Two Linear Equations Can Represent a Line: 2:08:20
- Note 2: Line of Intersection from Two Parametric Planes: 2:16:18
- Example 8: Distance from a Point to a Plane: 2:19:55
- Example 9: 2:30:45
- Example 10: 2:38:04
- Exercise 1: 2:55:19
- PDF video notes: https://1drv.ms/b/s!As32ynv0LoaIiecyXiOIVbA_nizjLQ
- HIVE video notes: https://peakd.com/hive-128780/@mes/equations-of-lines-and-planes
- Video sections playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0FO3u0IupqllNNkZTsffpIV
- Vectors and the Geometry of Space video series: https://www.youtube.com/playlist?list=PLai3U8-WIK0FjJpwnxwdrOR7L8Ul8VZoZ
Related Videos:
Discovery Project: The Geometry of a Tetrahedron: https://peakd.com/hive-128780/@mes/discovery-project-geometry-of-a-tetrahedron
The Cross Product: https://peakd.com/hive-128780/@mes/vectors-and-the-geometry-of-space-the-cross-product
The Dot Product: https://peakd.com/hive-128780/@mes/vectors-and-the-geometry-of-space-the-dot-product
Complex Numbers as Rotation Matrices: https://peakd.com/hive-128780/@mes/complex-numbers-as-rotation-matrices
Vectors: https://peakd.com/hive-128780/@mes/vectors-and-the-geometry-of-space-vectors
3D Coordinate Systems: https://peakd.com/hive-128780/@mes/vector-space-and-geometry-3d-coordinate-systems .
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Shifted Conics: Parabolas
In this video I go over further into shifted conics and this time shift Parabolas. The procedure for shifting parabolas is the same as that for ellipses, which I covered in my earlier video. This is done by simply replacing x and y with (x – h) and (y – k). This means that in order to obtain the basic x and y values, we need to add h to the horizontal component and k to the vertical component. Thus we effectively shift the function horizontally and vertically. For the parabola y = ax^2, with the vertex at the origin, this can be shifted so that the vertex is (h, k) by re-writing the formula (y – k) = a(x – h)^2 or y = a(x – h)^2 + k. Although this is very similar to the case for an ellipse, it is nonetheless important to understand this concept through applying it to different functions, such as in this case a parabola, so make sure to watch this video!
Download the notes in my video: https://1drv.ms/b/s!As32ynv0LoaIh5Vqjg-jPzl6_SRXlw
View video notes on the Hive blockchain: https://peakd.com/mathematics/@mes/shifted-conics-parabolas
Related Videos:
Shifted Conics: Ellipses (and Circles): https://youtu.be/ZqtUfaQbj7U
Equation of a Circle and it's proof: http://youtu.be/xMXYJ9UeF4I
Conic Sections: Hyperbolas: Example 2: Vertical Hyperbola: https://youtu.be/zBNSTkoEzFI
Conic Sections: Hyperbola: Definition and Formula: https://youtu.be/UBIHovXNV9U
Conic Sections: Parabolas: Definition and Formula: https://youtu.be/kCJjXuuIqbE
Conic Sections: Ellipses: Definition and Derivation of Formula (Including Circles): https://youtu.be/9dETsJ2tz_M
Hyperbola - Definition and derivation of the equation: x^2/a^2 - y^2/b^2 = 1: http://youtu.be/Y6iYC4VEAi0
Hyperbola Examples: http://youtu.be/LvccUMFrY3k .
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🔬#MESExperiments 30: Magnetic Spinning Top Aligns Opposite of Both Gravity and Magnetic Attraction
In #MESExperiments 30 I continue with the the GyroTop magnetic spinning top from Experiments 28 and 29, and this time showcase that it behaves the same way with gravity as it does with magnetic attraction. I demonstrate this by turning the GyroTop side-ways and then slowly extending it outwards so that the magnetic attraction becomes less while the downward force of gravity plays a more dominant role. One would expect that the spinning top would fall downwards as it extends further away from the magnetic but counterintuitively it rises upwards against gravity!
This is similar to its tendency to move opposite of the magnetic attraction, and the direction the GyroTop points can be considered as opposite of the vector sum of both gravity and magnetism. Truly remarkable stuff!
The timestamps of key moments of the experiment are listed below.
1. Spinning up the GyroTop: 0:00
2. Gradually Extending the GyroTop from a Horizontal Position: 0:33
3. Gradually Pulling Back the GyroTop from its Risen Position: 1:14
4. Extending the GyroTop Even Further: 1:21
5. Pulling Back the GyroTop Again: 1:45
6. GyroTop Hits the Magnetic Base: 1:57
Stay tuned for #MESExperiments 31...
View video notes on the Hive blockchain: https://peakd.com/hive-128780/@mes/mesexperiments-30-magnetic-spinning-top-aligns-opposite-of-gravity-and-magnetism
View more experiments!
- #MESExperiments video series: https://peakd.com/mesexperiments/@mes/list
- DRAFT #MESExperiments video series: https://mes.fm/experiments-draft
Related Videos:
#MESScience video series: https://mes.fm/science-playlist
#AntiGravity video series: https://peakd.com/antigravity/@mes/series
#FreeEnergy video series: https://mes.fm/freeenergy-playlist
🔬#MESExperiments 28: Magnetic Spinning Top Aligns Opposite of Magnetic Attraction: https://peakd.com/hive-128780/@mes/mesexperiments-28-magnetic-spinning-top-aligns-opposite-of-magnetic-attraction
🔬#MESExperiments 27: Spin Friction Can Make Gyroscope Rise from Steep 70° Angle (No Casing Rotation)
🔬#MESExperiments 25: Increasing Spin Friction Can Make Gyroscopes Rise Much Faster: https://peakd.com/hive-128780/@mes/mesexperiments-25-increasing-spin-friction-can-make-gyroscopes-rise-much-faster
🔬#MESExperiments 13: Steepest Gyroscope Rising Experiment Ever! 74 Degrees from the Vertical: https://peakd.com/mesexperiments/@mes/mesexperiments-13-steepest-gyroscope-rising-experiment-ever-74-degrees-from-the-vertical-magic
🔬#MESExperiments 3: Gyroscopes Exert 'Inertial Forces' With ZERO Loss of Spin Rate: https://peakd.com/mesexperiments/@mes/mesexperiments-3-gyroscopes-exert-inertial-forces-with-zero-loss-of-spin-rate-freeenergy
🔥#MESScience 1: How Does a Powerball Gyroscope Work? + Gyros Are Inverted Pendulums: https://peakd.com/messcience/@mes/messcience-1-how-does-a-powerball-gyroscope-work-gyros-are-inverted-pendulums
☝#AntiGravity Part 6: Objects in Rotation Defy ‘Mainstream’ Physics + MES Duality Concept: https://ecency.com/antigravity/@mes/antigravity-part-6-video-1-objects-in-rotation-defy-mainstream-physics-mes-duality-concept .
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MES Conclusions: No Evidence of COVID-19 "Virus" Isolation
In this video I provide my conclusions after reviewing the main paper on COVID-19 "virus" isolation and conclude that there was very little "isolating" being done. The paper involves a handful of patients assumed to have a "viral" infection of "unknown cause". Samples taken from the patients were assumed to contain a "virus". Genetic sequencing of non-isolated particles came up with a list of molecules (aka a "genome") and after comparing with other genomes, a "new virus" was "identified". The patient samples were then heavily processed in a complex procedure involving many different chemicals, cells, heavy metals, and then fixed with preservatives in a resin block. Optical light microscopy shows unclear images of supposed "cytopathic effects", and alleged "mock-infected" controls were not explained in any detail. High powered Electron microscope images of the heavily processed material showed blurry images of dead particles, which the authors assume to be "virions". Besides the need to examine the mainstream genetic sequencing science in depth, there was very little evidence in the paper of anything significant, and much less a "disease-causing invisible particle that can jump from person to person".
Some interesting "coincidences" that occurred during this COVID-19 psychological operation include China's new strict vaccine law coming into effect on December 1, 2019, the same day that the alleged "Patient Zero" developed symptoms. And then on New Year's Eve December 31, 2019, the China CDC sends their rapid response team to Wuhan... And "wuhan" itself can be rearranged to form the word "human", aka a human-contrived psychological operation.
And of course the paper ends by foreshadowing the global mind-control propaganda that was to follow soon after urging the public to "prevent, control, and stop the spread of 2019-nCoV"...
The paper is listed below:
- A Novel Coronavirus from Patients with Pneumonia in China, 2019
- Authors: Na Zhu et al
- Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
- https://www.nejm.org/doi/full/10.1056/NEJMoa2001017
The timestamps of key parts of the video are listed below:
- MES Conclusions: 0:00
- December 1, 2019: China Vaccine Law and "Patient Zero": 1:40
- MES Anagram: Wuhan = Human: 2:11
This video is taken from my earlier video listed below:
- 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
- Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx
Related Videos:
🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist .
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MES Summary of Main Paper on COVID-19 "Virus" Isolation
In this video I go over a summary of the main paper on COVID-19 "virus" isolation while only focusing on the paper's main claim of a "new disease-causing virus". ChatGPT had selected this paper as being the main paper on COVID-19 "virus" isolation and indeed this was the original and highly cited report by the China CDC Wuhan investigation team. I summarize all the key methods and claimed evidence provided in the report such as the chest radiographs, the heavily processed cell cultures with assumed "infection" from patient sample supernatants, and electron microscope images of dead heavy metal stained supernatants and cell cultures. I also include the claims of initial sequencing of "new viral RNA" in the supernatants, and the final computer generated "new virus genome". The paper and methods are very obscure and there is little evidence of anything significant, much less a "disease-causing new virus".
The paper is listed below:
- A Novel Coronavirus from Patients with Pneumonia in China, 2019
- Authors: Na Zhu et al
- Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
- https://www.nejm.org/doi/full/10.1056/NEJMoa2001017
The timestamps of key parts of the video are listed below:
- MES Summary: 0:00
- ChatGPT on the Main COVID-19 "Virus" Isolation Paper: 0:42
- Chest Radiographs of a Wuhan Pneumonia Patient: 1:47
- 4 Airway Samples Taken and "Unknown Viral RNA" Discovered: 2:39
- Cell Culture Supernatant Assumed to "Isolate the Virus": 4:01
- "Mock-Infected" vs "New Virus Infected" Cell Cultures: 6:41
- Supernatant and Cell Cultures were then "Fixed" with Chemical Preservatives: 7:18
- Electron Microscope Images of Heavy Metal Stained Supernatant and Cell Cultures: 9:11
- RNA Extracted Assumed to be from a "Virus": 10:33
- "New Virus" Genome and Phylogenetic Tree: 11:27
This video is taken from my earlier video listed below:
- 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
- Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx
Related Videos:
🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist .
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Review of COVID-19 "Virus" Isolation Paper: Discussion Section
In this video I go over the discussion section of the main paper on COVID-19 "virus" isolation. The discussion section outlines the authors' alleged claims of a "new virus" and the follow-ups suggested for supposedly tracking and stopping the "spread" of this alleged "virus" and its associated alleged disease. The new "virus" is claimed to have been identified via whole-genome sequencing, direct PCR, and cell cultures. The genome was then submitted to the GISAID and compared with other "coronaviruses" before claiming it is separate from the other previously known "viruses". The electron microscope images of blurry particles were assumed to be this new "virus". Specific sequences from the alleged "new virus genome" were then selected and submitted to the WHO for "detecting" this new "virus" in the general population; which the authors state 830 people in China have already tested "positive" as of the paper's publishing. The authors admit their paper doesn't fulfill Koch's postulates and instead claim their study only "implicates" the "new virus" in the Wuhan "outbreak".
The paper highlights additional experiments to confirm this claim of a "disease-causing new virus". These experiments include antigen detection in the lung tissue of patients, antibody detection in the blood of patients, and animal (monkey) experiments to demonstrate that the "virus" actually causes disease. Finally the paper concludes with the now infamous psychological mind control operation cliché of the alleged importance to "prevent, control, and stop the spread of 2019-nCoV"...
The paper is listed below:
- A Novel Coronavirus from Patients with Pneumonia in China, 2019
- Authors: Na Zhu et al
- Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
- https://www.nejm.org/doi/full/10.1056/NEJMoa2001017
The timestamps of key parts of the video are listed below:
- "New Virus" Detected via Sequencing, PCR, and Culture: 0:00
- Direct PCR: 1:12
- Next-Generation Sequencing and Bioinformatics Accelerating Detection of "Pathogens": 1:23
- Bioinformatics: 2:32
- Electron Microscopy and Genome Sequencing Visualized and Detected the "New Virus": 2:52
- Koch's Postulates, Asymptomatic Carriers: 4:43
- Additional Experiments Needed: Immunohistochemical Analysis, Animal Experiments: 6:54
- 2019-nCoV Antigen, Immunohistochemical (IHC) Analysis, Seroconversion: 7:58
- Summary of Paper Discussion: 10:52
This video is taken from my earlier video listed below:
- 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
- Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx
Related Videos:
🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist .
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COVID-19 Paper Results: Genetic Characterization of the "Virus"
In this video I go over the results section on the main COVID-19 "virus" isolation paper and look at the section that supposedly provides the genome and phylogenetic tree of this "new virus". De novo sequencing was performed on airway fluid samples and cell culture supernatants from the three Wuhan patients, and supposedly a "novel coronavirus" was identified in all three patients. These sequences were sent to the GISAID global flu "virus" database and compared with previous "virus" genomes. The 3 "virus" genomes had 86.9% similarity with previous "bat coronaviruses", but had less than 90% similarity of supposed specific genes involved in "viral" replication. Since they were less than 90% similar, this "new virus" was given its own clade on the phylogenetic tree.
Interestingly, the paper refers to the cell culture supernatants as "virus isolates", even though no common sense isolation was performed. It is clear that no actual "virus" is being observed, but it still raises the question of what these "genomes" refer to, how they are obtained, and what is the reasoning for grouping them in their respective clades.
The paper is listed below:
- A Novel Coronavirus from Patients with Pneumonia in China, 2019
- Authors: Na Zhu et al
- Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
- https://www.nejm.org/doi/full/10.1056/NEJMoa2001017
The timestamps of key parts of the video are listed below:
- Genetic Characterization of the "Virus": 0:00
- De Novo Sequencing: 0:48
- Complete Genome Sequences Somehow Obtained: 1:19
- Global Initiative on Sharing All Influenza Data (GISAID), Influenza: 4:34
- 2019-nCoV Supposedly forms a Distinct Clade: 5:24
- Replicase Domains (ORF 1ab): 7:00
- New BetaCoV Alleged Genome and Phylogenetic Tree: 7:12
- Summary of the Genetic Characterization of the "Virus": 9:16
This video is taken from my earlier video listed below:
- 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
- Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx
Related Videos:
🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist .
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COVID-19 Paper Results: Detection and Isolation of a "New Virus"
In this video I go over the main COVID-19 "virus" isolation paper and look at the results section titled "Detection and Isolation of a Novel Coronavirus". While the title of the section suggests a "new virus" was discovered, the actual methodology used to come to this conclusion is lacking, to say the least. The usual virology methodology was used, which was take RNA from a handful of patients, assume it's from a "virus", and then use genetic sequencing technology to somehow obtain 20,000 short RNA sequences and compare with known "viral sequences" in an online database. Then PCR tests were performed with high cycle threshold values to detect common sequences shared among a group of "beta-coronaviruses". Growing cell cultures on human airway cells, monkey kidney cells, and liver cancer cells were assumed to be the same as "isolating" the "virus". The cell cultures were then observed for "cytopathic effects" and compared with a "mock infected" cell culture (with no detail given of this "mock infected" culture), and the images provided of the cytopathic effects were unclear, and insignificant in my opinion. Finally, the electron micrographs were provided of the heavily processed cell cultures and cell culture supernatants, supposedly showing the "virus" particles along with their "solar corona" spikes. Again, to me this is not strong evidence of "viruses", nor even of what exactly these particles are or what they do or can do, if anything.
The paper is listed below:
- A Novel Coronavirus from Patients with Pneumonia in China, 2019
- Authors: Na Zhu et al
- Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
- https://www.nejm.org/doi/full/10.1056/NEJMoa2001017
The timestamps of key parts of the video are listed below:
- Paper Results: Detection and Isolation of a "Novel Coronavirus": 0:00
- HCoVs, RNA Template to Clone, Viral Reads: 2:46
- PCR to Detect Known "Viral RNA Regions": 3:40
- PCR Cycle Threshold (Ct), VERO Cell Lines, Cloning Cell Lines: 4:35
- Cytopathic Effects of "Infected" and Mock-Infected Cell Cultures: 9:20
- Mock-Infected, Cilium Beating: 10:47
- Electron Micrographs of Alleged "Virus" Particles: 11:17
- Pleomorphism, Solar Corona, Extracellular "Virus" Particles, "Virus" Inclusion Bodies: 13:49
- Summary of "New Virus" Detection and Isolation: 16:36
This video is taken from my earlier video listed below:
- 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
- Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx
Related Videos:
🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist .
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Review of COVID-19 Paper Results: Patients
In this video I go over the results section of the main paper on COVID-19 "virus" isolation and look at the first part which is on the patients used in the paper. Three adult patients with severe pneumonia (inflammation of the lungs) were admitted in a Wuhan hospital in December 2019. Patients 1 and 3 survived but Patient 2 died after being put on a mechanical ventilator (it is not clear if the other patients were also put on a mechanical ventilator or not). No biopsy or tissue samples were obtained from any of the samples. CT scans were provided for Patient 2 and showed cloudy regions termed "bilateral fluffy opacities". Personally, there was nothing significant with these 3 patients, besides possibly the use of mechanical ventilation in making the patients pneumonia worse...
The paper is listed below:
- A Novel Coronavirus from Patients with Pneumonia in China, 2019
- Authors: Na Zhu et al
- Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
- https://www.nejm.org/doi/full/10.1056/NEJMoa2001017
The timestamps of key parts of the video are listed below:
- Paper Results: Patients: 0:00
- Tomography, Computed Tomography (CT) Scan, Radiography: 1:22
- Patient 2 Received Mechanical Ventilation and Died: 3:03
- Respiratory Distress, Ventilator, Biopsy: 3:42
- Chest Radiographs: 4:23
- Trachea, Intubation, Bilateral Fluffy Opacities: 5:48
- Summary of Patients Results: 7:43
This video is taken from my earlier video listed below:
- 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
- Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx
Related Videos:
🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist .
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Shifted Conics: Hyperbolas
In this video I shift more conic sections and this time shift Hyperbolas. Once again this procedure is the same as in my earlier videos on shifting ellipses (and circles) and parabolas. Nonetheless it is important to see the same procedure applied to different functions. Shifting a parabola with the center at the origin so that the center is now at the point (h , k) requires switching every (x , y) point on the original hyperbola with (x – h, y – k). Thus to obtain the new (x , y) values we need to add h to every x – h value, and k to every y – k value. In other words, we shift every point horizontally by h and vertically by k. This is a great video to reiterate the concept of shifting functions in general so make sure to watch this video!
Download the notes in my video: https://1drv.ms/b/s!As32ynv0LoaIh5YHzNNtJk4zVRcWHQ
View video notes on the Hive blockchain: https://peakd.com/mathematics/@mes/shifted-conics-hyperbolas
Related Videos:
Shifted Conics: Parabolas: https://youtu.be/UlrAkAW3T8Y
Shifted Conics: Ellipses (and Circles): https://youtu.be/ZqtUfaQbj7U
Equation of a Circle and it's proof: http://youtu.be/xMXYJ9UeF4I
Conic Sections: Hyperbolas: Example 2: Vertical Hyperbola: https://youtu.be/zBNSTkoEzFI
Conic Sections: Hyperbola: Definition and Formula: https://youtu.be/UBIHovXNV9U
Conic Sections: Parabolas: Definition and Formula: https://youtu.be/kCJjXuuIqbE
Conic Sections: Ellipses: Definition and Derivation of Formula (Including Circles): https://youtu.be/9dETsJ2tz_M
Hyperbola - Definition and derivation of the equation: x^2/a^2 - y^2/b^2 = 1: http://youtu.be/Y6iYC4VEAi0
Hyperbola Examples: http://youtu.be/LvccUMFrY3k .
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COVID-19 Paper: RNA is Assumed to be from a "Virus"
In this video I go over the main assumption in the main paper on COVID-19 "virus" isolation, and that is the RNA extracted from patient and cell culture supernatants is assumed to be "viral". It took me a long time before realizing this since the paper does not state this directly. Instead, I had to ask ChatGPT to clarify a paragraph from the paper, as well as double-checking with the mainstream science literature. And indeed this is a key assumption made in virology in general. Ribonucleic acid (RNA) is supposedly the genetic makeup of the new SARS-CoV 2 "virus" which supposedly causes "COVID", so when a "viral infection" is suspected, then typically the assumption is that RNA found is from this "virus".
ChatGPT also outlines several methods to "confirm" this assumption, such as using PCR to detect specific RNA sequences, comparing the sequences with known "viral sequences", and "immunological assays". These assays use "antibodies" (or immune system "defense proteins") that are supposedly specific to and bind to proteins (called antigens) on the "virus"; thus allowing for indirect identification of this "virus".
All of this is interesting, yet it still remains highly questionable that a bunch of nucleotide sequences (aka RNA) are somehow assumed to be "infectious disease causing particles that can infect and replicate through a host and spread to other individuals (and even shut down the entire world)"...
The paper is listed below:
- A Novel Coronavirus from Patients with Pneumonia in China, 2019
- Authors: Na Zhu et al
- Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
- https://www.nejm.org/doi/full/10.1056/NEJMoa2001017
The timestamps of key parts of the video are listed below:
- RNA Assumed to be from a "Virus": 0:00
- RNA extracted from Airway Fluid Samples and Culture Supernatants: 0:22
- Asking #ChatGPT to Explain Paragraph: 0:48
- #ChatGPT: Assumption is RNA is from a "Virus": 1:41
- Summary of RNA "Virus" Assumption: 3:21
This video is taken from my earlier video listed below:
- 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
- Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx
Related Videos:
🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist .
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Review of COVID-19 Paper Methods: Sequencing the "Virus"
In this video I go over the main COVID-19 "virus" isolation paper's methods on the techniques used in sequencing this "new virus". The first step in sequencing the "virus" is assuming that the RNA found in the patient airway fluid samples and cell culture supernatants somehow belong to a "virus". After this assumption, a combination of Illumina sequencing and nanopore sequencing were used to somehow produce a "virus genome". After this, PCR tests were designed with specific primers to test for this new "virus". The "virus" sequences were then compared with reference sequences. And finally, statistical analysis was performed on determining the likely evolutionary history of the "virus" genome. Essentially, the paper outlines a lot of obscure computer and statistical techniques to form long genetic sequences from RNA assumed to be from a "virus". Truly bizarre "science"...
The paper is listed below:
- A Novel Coronavirus from Patients with Pneumonia in China, 2019
- Authors: Na Zhu et al
- Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
- https://www.nejm.org/doi/full/10.1056/NEJMoa2001017
The timestamps of key parts of the video are listed below:
- Paper Methods: Sequencing the "Virus": 0:00
- RNA as a Template to Clone and Sequence Genome: 0:37
- Illumina Sequencing, Nanopore Sequencing: 1:13
- Sequence Reads Assembled into Contig Maps: 1:46
- Contig, Contiguous, Contig Map: 2:00
- Primers Designed for PCR to Fill Genome Gaps: 3:11
- Primer, 5' or 3', Complementary DNA (cDNA), Sanger Sequencing: 3:44
- Multiple-Sequence Alignment of New "Virus": 6:19
- Multiple-Sequence Alignment, Phylogenetics, Bootstrap Replicates: 6:41
- Summary of Sequencing the "Virus": 7:30
This video is taken from my earlier video listed below:
- 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
- Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx
Related Videos:
🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist .
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Review of Paper: Electron Microscope Preparation for COVID-19 "Virus"
In this video I go further into the main paper on COVID-19 "virus" isolation and this time review the paper's methods on transmission electron microscopy (TEM) preparation. TEM involves firing a beam of electrons through an ultrathin section and the resulting interactions with the sample forms an image that can then be made visible through fluorescent screens or other methods. But to get to this point, the sample is typically first mixed with many heavy metal chemicals and embedded into a resin block. This means that any biological samples will be dead and heavily processed.
For this paper, the first sample prepared was the supernatant of the "infected" human airway cell cultures that showed "cytopathic effects" (CTE), and was then "virus inactivated" with a chemical preservative and ultracentrifuge at around 100,000 RPM. The resulting sedimented "virus" particles was then heavy metal stained on film-coated metal grids.
The second sample was the "infected" airway cell cultures (that showed CTE) themselves, and they were "fixed" with heavy metal preservatives, dehydrated with alcohol, and then embedded in a resin block before being cut to tiny nanometer slices for TEM.
This is a very complex process to somehow see dead particles assumed to be "virions"...
The paper is listed below:
- A Novel Coronavirus from Patients with Pneumonia in China, 2019
- Authors: Na Zhu et al
- Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
- https://www.nejm.org/doi/full/10.1056/NEJMoa2001017
The timestamps of key parts of the video are listed below:
- Paper Methods: Electron Microscope Preparation: 0:00
- Virus Inactivation, Paraformaldehyde, Fixation: 0:49
- Ultracentrifuge, Transmission Electron Microscopy (TEM), Heavy Metals: 1:41
- Fluorescence, Electrons, Electromagnetic Radiation (EMR), Aether: 2:29
- TEM Schematic: 3:22
- Enriched Supernatant Was Negatively Stained: 4:18
- Negative Stain, Staining, Film-Coated Grids, Hydrophilic, Formvar Grid: 5:06
- Fixing Airways Cells Showing Cytopathic Effects: 6:22
- Glutaraldehyde, Osmium Tetroxide, Grade Ethanol: 6:54
- PON812 Resin, Viscosity, Embedding: 7:31
- Ultrathin Sections Cut from Resin Block: 8:27
- Nanometers, Uranyl Acetate, Lead Citrate: 8:51
- Summary of Electron Microscope Preparation: 9:10
This video is taken from my earlier video listed below:
- 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
- Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx
Related Videos:
🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist .
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Review of Paper Methods: Isolating the COVID-19 "Virus"
In this video I go over further into the main paper on COVID-19 "virus" isolation, and this time review the paper's methods in isolating the "novel virus". The stated methods involved first collecting the lung fluid samples in a plastic cup, and then chemical preservatives (PBS) or fetal bovine serum (FBS) were added. The samples were then centrifuged and the resulting supernatant was assumed to somehow contain the "virus" and used to "infect" human airway cell cultures obtained from other lung cancer patients. Then the cultures were "incubated" or grown for 2 hours at 37°C, and then diluted to a new cell culture. This procedure was done 3 times total and each time the cell cultures were monitored using optical light microscopy for "cytopathic effects" (or cell breakdown / death). Cultures showing cytopathic effects were then prepared for electron microscopy. No information on the procedure for controls were presented. And no explanation as to why the centrifuged supernatant of the patient lung fluid samples were assumed to contain the "virus".
The paper is listed below:
- A Novel Coronavirus from Patients with Pneumonia in China, 2019
- Authors: Na Zhu et al
- Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
- https://www.nejm.org/doi/full/10.1056/NEJMoa2001017
The timestamps of key parts of the video are listed below:
- Paper Methods: Isolating the "Virus": 0:00
- Viral Transport Medium (VTM), Acids and Bases : 1:14
- Fetal Bovine Serum (FBS): 3:08
- Samples Centrifuged, Supernatant Use to Infect: 3:57
- Centrifugation, Supernatant, Cancer: 4:31
- Cells Grown on Plastic Surface: 7:01
- Plastic substrate, Expanding Cells, Passage-1 Cells, Plating: 7:29
- Cells Cultures Grown in Air-Liquid Interface: 9:42
- Air-Liquid Interface (ALI), Cellular Differentiation: 10:04
- Stem Cells, Respiratory Epithelium, Cilia: 12:57
- Apical Cells Washed with PBS: 14:14
- Apical, Microlitre: 15:08
- MES Note: Where is the "Virus"?: 16:13
- 2-hour Incubation, Unbound "Virus" Washed Away: 16:40
- Incubator, 37° C, Serial Passage: Cultural Dilution: 18:16
- Cells Monitored for Cytopathic Effects: 19:12
- Light Microscopy, Cytopathic Effect: 19:54
- Summary of Isolating the "Virus": 20:34
This video is taken from my earlier video listed below:
- 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
- Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
- Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx
Related Videos:
🔥#MESScience: MES Science Tutorials: https://mes.fm/science-playlist .
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