proliferation assay

bellasmithPublished: November 27, 2017
Published: November 27, 2017

SIAT® DC-T cell proliferation assay uses Flow Cytometry to detect T cell proliferation after co-culture with loaded DCs, which is extremely sensitive and accurate. Specifically, T cells are labeled with 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE) dye. Those that proliferate in response to peptide antigen presented by DCs show a CFSE dye dilution, which can be measured directly by Flow Cytometry. In combination with detection of cell surface markers, the percentage of proliferating CD4+ cells, as well as the detailed phenotype of T cell responses are all monitored. Below is a brief description of our workflow for SIAT® DC-T cell proliferation assay service.

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proliferation assay